Photoaffinity labeling of the ryanodine receptor/Ca2+ release channel with an azido derivative of ryanodine.
نویسندگان
چکیده
منابع مشابه
Modification of ryanodine receptor/Ca2+ release channel with dinitrofluorobenzene.
Modification of the ryanodine receptor (RyR)/Ca(2+) release channel with 2,4-dinitrofluorobenzene (DNFB) indicated that two classes of amino group interact with the reagent, as can be distinguished on the basis of their reactivity/accessibility and the effects on ryanodine binding and single channel activities. One group interacted very rapidly (t(1/2)<30 s) at 25 degrees C with low concentrati...
متن کاملPhotoaffinity labeling of the Klenow fragment with 8-azido-dATP.
The photoaffinity compound 8-azido-dATP was used as a probe for the deoxyribonucleoside triphosphate-binding site of the large fragment of DNA polymerase I. Azido-dATP specifically modified a saturable binding site within the Klenow fragment, and each of the four natural deoxyribonucleoside triphosphate substrates competed with labeling at this site in proportion to its binding constant, as pre...
متن کاملRedox regulation of the ryanodine receptor/calcium release channel.
The RyR (ryanodine receptor)/calcium release channel contains a number of highly reactive thiol groups that endow it with redox sensitivity. In general, oxidizing conditions favour channel opening, while reducing conditions have the opposite effect. Thiol modification affects the channel sensitivity to its principal effectors, Ca2+, Mg2+ and ATP, and alters RyR protein interactions. Here, we gi...
متن کاملNitric oxide and the ryanodine receptor Ca-release channel.
Nitric Oxide and the Ryanodine Receptor Ca-Release Channel To the Editor: I have read with interest the recent review on the role of S-nitrosylation in cardiovascular signaling, in which Lima et al1 provide a very useful digest on the current status of research in this important but burgeoning field of cardiovascular physiology. On the other hand, I believe that one of the many functions of a s...
متن کاملPhotoaffinity labeling of the mitochondrial oxoglutarate carrier by azido-phthalonate.
The effect of azido-phthalonate, a photoreactive analogue of oxoglutarate, on the transport of oxoglutarate was investigated in proteoliposomes reconstituted with the purified oxoglutarate carrier. In the dark, azido-phthalonate inhibits the reconstituted oxoglutarate/oxoglutarate exchange in a competitive manner with a Ki of 0.38 mM. Upon photoirradiation, the inhibition of the oxoglutarate ex...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)36799-6